TAP medium
from Gorman, D.S., and R.P. Levine (1965) Proc. Natl. Acad. Sci. USA 54, 1665-1669.
This is probably the most widely-used medium at present for experimental work.
Make the following stock solutions:
1. TAP salts
|
NH4Cl |
|
15.0 g |
|
MgSO4 . 7H2O |
|
4.0 g |
|
CaCl2 . 2H2O |
|
2.0 g |
|
water to 1 liter |
|
|
2. phosphate solution
|
K2HPO4 |
|
28.8 g |
|
KH2PO4 |
|
14.4 g |
|
water to 100 ml |
|
|
3. Hutner´s trace elements (follow this link)
To make the final medium, mix the following:
2.42 g Tris
25 ml solution #1 (salts)
0.375 ml solution #2 (phosphate)
1.0 ml solution #3 (trace elements)
1.0 ml glacial acetic acid
water to 1 liter
For solid medium, add 15 g agar per liter
Autoclave.
For Tris-minimal medium omit the acetic acid and titrate the final solution to pH 7.0 with HCl